ABL :: Liposomes Example Page

ABL standard fluorescent liposomes contain either Calcein or fluorescein and are approx 200nm in in diameter. Further information including full dequenching assay and precise fluorophore concentrations are with the material in the form of product sheet.. These fluorophores are encapsulated at self-quenching concentrations so that fluorescent signal is extinguished. Upon trigger dequenching occurs to produce a large fluorescent signal amplification. This signal amplification feature makes them useful for standard fluorescent labelling applications, assays to determine membrane disrupting properties of compounds or diagnostic application when used with cytolytic peptides.

Standard Fluorescent Liposomes ABL standard fluorescent liposomes contain either Calcein or fluorescein and are approx 200nm in in diameter. Further information including full dequenching assay and precise fluorophore concentrations are with the material in the form of product sheet.. These fluorophores are encapsulated at self-quenching concentrations so that fluorescent signal is extinguished. Upon trigger dequenching occurs to produce a large fluorescent signal amplification. This signal amplification feature makes them useful for standard fluorescent labelling applications, assays to determine membrane disrupting properties of compounds or diagnostic application when used with cytolytic peptides.
Product information Product: Calcein Liposomes Lipid Composition: Egg Phosphatidylcholine/Cholesterol Physical specifications Appearance: Orange suspension. Solubility: Particles suspend Phosphate buffered saline while the payload is soluble. Storage: cool and away from light. DO NOT FEEZE Analytical dataMean diameter: 210nm measured by PCS . Molar ratio 2: 1 Egg Phosphatidylcholine:Cholesterol. Lipid concentration by Stewart assay: 3 mg/ml
	Dequenching assay:Assay: 2ml isotonic buffer + 2µl liposomes. Triton added (10µl of 10% solution) to illustrate full signal release. Trace shown left. Excitation and emission wavelengths were 490 and 520nm.
Comments: It is recommended to use an isotonic buffer for dilution of liposomes to prevent undue release of fluorophore. Warning: All our products are sold for Laboratory use only and not for drug, household or other use. The product is not for use in humans.
Other Fluorophores, Lipid ratio`s and Compositions are available please Contact Us See also our Lipid Mixtures for Liposome preparation

Standard Fluorescent Liposomes